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Mouse Anti-SMAD7 antibody
单克隆  |   CloneNo.: 5H11  |   SKU:bsm-33869M

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货号:bs-3351R
¥1280
订购号:bsm-33869M
¥1138.00-3200.00
货期:现货
Mouse Anti-SMAD7 antibody
反应物种(预测)

Mouse,Rat

产品应用(已验证)

WB,IHC

推荐稀释比例

WB=1:500-2000,IHC-P=1:400-800,

研究领域

肿瘤,细胞生物,信号转导,细胞凋亡,生长因子和激素,激酶和磷酸酶

标签

Array

  • Sample:
    Lane 1: Human Hela cell Lysates
    Lane 2: Human 293T cell Lysates
    Primary: Anti-SMAD7 (bsm-33869M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 46kDa
    Observed band size: 46kDa
  • Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (bsm-33869M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (bsm-33869M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

产品信息

RRID:RRID
产品名称:Mouse Anti-SMAD7 antibody
别名: MAD (mothers against decapentaplegic Drosophila) homolog 7; MAD; Mad homolog 7; MAD mothers against decapentaplegic homolog 7; MADH 7; MADH 8; MADH8; Mothers Against Decapentaplegic Drosophila Homolog of 7; Mothers against decapentaplegic homolog 7; Mothe
中文名称:Smad7单克隆抗体
英文名称:Mouse Anti-SMAD7 antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human SMAD7
SWISS:O15105
Gene ID :4092
Human Gene ID:4092

产品介绍

The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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