Histostain-SP (Streptavidin-Peroxidase) and Histostain-SAP (Streptavidin-Alkaline Phosphatase) immunohistochemical staining kits employ the LAB-SA (Labeled [strept]Avidin-Biotin) method. For Histostain-SP Kits, DAB or AEC chromogen/substrates generate a colored precipitate around the target antigen in human samples. DAB (brown) is approximately twice as sensitive as AEC (reddish). For Histostain-SAP Kits, AP-Red or BCIP/NBT chromogen/substrates generate a colored precipitate around the target antigen in human samples. BCIP/NBT (dark blue/purple) is about twice as sensitive as AP-Red (red/magenta).
Reactivity: Rabbit primary antibody
Applications: Immunohistochemistry Detection
即用型SP(Streptavidin-Peroxidase)免疫组化检测试剂盒是利用LAB-SA第二代技术生产的免疫组化检测试剂,最大特点是高灵敏度,低背景染色,直接滴加,操作方便,孵育时间短,适用于冷冻切片、石蜡切片及固定的细胞涂片。
可用于检测兔来源的一抗
SP试剂盒内容
无色试剂:内源性过氧化物酶阻断剂
试剂A:封闭用正常山羊血清工作液
试剂B:生物素标记二抗工作液:生物素标记羊抗兔IgG
试剂C:辣根酶标记链酶卵白素工作液
温馨提示:
1. 本系列检测试剂盒适用于绝大数组织,但对于内源性生物素含量比较丰富的组织(如肾脏、肝脏等),应选用非生物素检测系统,或用avdin封闭。
2. 工作液:如以每张切片加入1滴(约50μl)计算,3ml可做60张切片,6ml可做120张切片。
相关二抗试剂盒
SP-0022(SP-9000):生物素标记山羊抗兔、大鼠、小鼠和豚鼠 IgG
SP-0023(SP-9001):生物素标记山羊抗兔IgG
SP-0024(SP-9002):生物素标记山羊抗小鼠 IgG