| Rabbit Anti-IGF 1 antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA,IP |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IP=1:20-100,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
免疫学,发育生物学,信号转导,生长因子和激素,糖尿病, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (bs-0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Human IGF1 protein (full length) Overexpression E.coli Lysate at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti-IGF 1 (bs-0014R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
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Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (bs-0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855139
产品名称：Rabbit Anti-IGF 1 antibody
别名： IBP1; IGF 1; IGF I; IGF-1;IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Full=Insulin-like growth factor I; Insulin Like Growth Factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor
中文名称：胰岛素样生长因子1抗体
英文名称：Rabbit Anti-IGF 1 antibody
中文别名：IGF-I;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human IGF-1
抗原表位：49-100/195
SWISS：P05019
Gene ID ：3479
Human Gene ID：3479
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
Function：The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthe
Subcellular Location：Secreted.
DISEASE：Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
Similarity：Belongs to the insulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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