| Rabbit Anti-p75 NGF Receptor antibody |
| 反应物种（预测） |
Mouse,Dog,Pig,Cow,Horse,GuineaPig |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test ,IF=1:100-500, |
| 研究领域 |
细胞生物,神经生物学,信号转导 |
| 标签 |
Array |
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Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- p75 NGF Receptor antibody (bs-0161R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control: (mo)nephrocyte(blue)
Isotype Control Antibody: Rabbit IgG -AF647(orange);
Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green).
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Blank control:Mouse brain.
Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (bs-0161R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10856008
产品名称：Rabbit Anti-p75 NGF Receptor antibody
别名： NGFR; Gp80 LNGFR; ngfrp75; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; NGF receptor; p75 ICD; CD271; CD271 antigen; Gp80 LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR;
中文名称：神经生长因子受体抗体
英文名称：Rabbit Anti-p75 NGF Receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜,细胞外基质
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human NGFR
抗原表位：301-400/427
抗原细胞定位：Cytoplasmic
SWISS：P07174
Gene ID ：4804
Human Gene ID：4804
The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis.
Function：Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Subunit：Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. In
Subcellular Location：Membrane; Single-pass type I membrane protein.
Post-translational modifications：N- and O-glycosylated.
Phosphorylated on serine residues.
Similarity：Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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