Rabbit Serum blocking reagent can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Application: Rabbit Serum blocking reagent is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.


封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭。可以封闭待检样本中与蛋白质非特异性结合的位点，另外还可以封闭样品中内源性的 Fc 片段结合位点，阻断抗体与样品中的 Fc 受体结合，从而降低背景，减少假阳性。在选择封闭血清时，要注意封闭用血清中不能含有目标蛋白，且与一抗来源不同，可用与二抗相同来源的封闭血清。正常兔血清被广泛应用于组织和细胞染色中非特异性抗体结合的阻断，以及抗体的其他应用。血清可直接用于阻断，或作为阻断缓冲液的成分。


使用方法

免疫组化： 用5-10%正常兔血清封闭（1:10-1:20倍稀释），室温孵育5-10分钟。清除血清，勿洗，滴加适当比例稀释的一抗或一抗工作液，37℃或室温孵育4℃过夜。

细胞涂片： 用5-10%正常兔血清封闭（1:10-1:20倍稀释），使用 PBS 稀释（ 0.01mol/L pH7.4），少量点滴：漠过细胞涂片即可.