| Rabbit Anti-CD20 antibody |
| 反应物种（预测） |
Dog,Cow,Rabbit |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /test, |
| 研究领域 |
肿瘤,免疫学,干细胞,细胞表面分子,细胞类型标志物(B lymphocytes marker),淋巴细胞,B-淋巴细胞, |
| 标签 |
Array |
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Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Bone (Mouse) Lysate at 40 ug
Primary: Anti-CD20 (bs-0080R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
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Blank control: U937 (blue).
Primary Antibody:Rabbit Anti- CD20 antibody(bs-0080R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0080R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
RRID：AB_10856459
产品名称：Rabbit Anti-CD20 antibody
别名： CD20_HUMAN; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; B1; CVID5; LEU-16; S7.
中文名称：CD20抗体
英文名称：Rabbit Anti-CD20 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CD20
抗原表位：133-182/297
抗原细胞定位：Cytoplasmic
SWISS：P11836
Gene ID ：931
Human Gene ID：931
CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.
Function：This protein may be involved in the regulation of B-cell activation and proliferation
Subcellular Location：Membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed on B-cells.
Post-translational modifications：Phosphorylated. Might be functionally regulated by protein kinase(s).
DISEASE：Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent
Similarity：Belongs to the MS4A family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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