| Rabbit Anti-Oct-3/Oct-4 antibody |
| 反应物种（预测） |
Dog,Pig,Cow |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,染色质和核信号,干细胞,表观遗传学, |
| 标签 |
Array |
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Tissue/cell: mouse tooth germ tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Oct-3/Oct-4 Polyclonal Antibody, Unconjugated(bs-1111R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Embryo (Mouse)Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) Cell Lysate at 40 ug
Hela(Human) Cell Lysate at 40 ug
Rsc96(Rat) Cell Lysate at 40 ug
293T(Human) Cell Lysate at 40 ug
SP2/0(Mouse) Cell Lysate at 40 ug
HL-60(Human) Cell Lysate at 40 ug
Primary: Anti-Oct-3/Oct-4 (bs-1111R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
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Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-3) Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-3) Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Oct-3 antibody (bs-1111R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10855418
产品名称：Rabbit Anti-Oct-3/Oct-4 antibody
别名： POU domain, class 5, transcription factor 1;Oct-3/4; Oct-4A; Oct4A; Oct 4A; Octamer-binding transcription factor 3; Octamer-4; Octamer-3; Oct-3; NF-A3; Otf-3; Otf3; MGC22487; Oct 3; Oct 4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4;
中文名称：胚胎干细胞关键蛋白抗体
英文名称：Rabbit Anti-Oct-3/Oct-4 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human OCT4
抗原表位：201-300/360
SWISS：P20263
Gene ID ：5460
Human Gene ID：5460
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Function：Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early emb
Subunit：Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2.
Subcellular Location：Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern.
Tissue Specificity：Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Post-translational modifications：Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK
Similarity：Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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