| Rabbit Anti-C5a anaphylatoxin antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
IF |
| 产品应用（可尝试） |
WB,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IF=1:100-500, |
| 研究领域 |
免疫学,神经生物学 |
| 标签 |
Array |
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Tissue/cell: Human laryngeal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C5b-9 Polyclonal Antibody, Unconjugated(bs-0324R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
RRID：AB_10857063
产品名称：Rabbit Anti-C5a anaphylatoxin antibody
别名： C5; C6; C7; C8; C9; Complement component 5; Complement component 6; Complement component 7; Complement component 8; Complement component 9; MAC; Membrane attack complex; TCC; Terminal complement complex.
中文名称：末端补体复合物抗体
英文名称：Rabbit Anti-C5a anaphylatoxin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Purified Human SC5b-9 Complex
抗原表位：701-755/1680
SWISS：P02748
Gene ID ：727
Human Gene ID：727
Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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