Rabbit Anti-MRC1 antibody |
反应物种(预测) |
Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep,GuineaPig |
产品应用(已验证) |
FCM |
产品应用(可尝试) |
WB,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /Test, |
研究领域 |
细胞生物,免疫学,转录调节因子,细胞膜受体,糖蛋白, |
标签 |
Array |
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Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Macrophage mannose receptor 1 antibody (bs-4727R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-Macrophage mannose receptor 1 antibody (bs-4727R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:RRID
产品名称:Rabbit Anti-MRC1 antibody
别名: Mannose Receptor; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor; Mannose receptor C type 1; MMR; MRC 1; MRC-1; MRC1_HUMAN; Macrophage mannose receptor 1; MRC1L1; C-type lectin domain
中文名称:巨噬细胞甘露糖受体 (CD206) 抗体
英文名称:Rabbit Anti-MRC1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human MRC1/CD206
抗原表位:201-300/1456
抗原细胞定位:Extracellular
SWISS:P22897
Gene ID :4360
Human Gene ID:4360
The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. This gene is in close proximity to MRC1L1. The gene loci including this gene, MRC1L1, as well as LOC340843 and LOC340893, consist of two nearly identical, tandemly linked genomic regions, which are thought to be a part of a duplicated region. [provided by RefSeq]
Function:Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Subcellular Location:Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein.
Similarity:Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
[SIMILARITY] Contains 1 ricin B-type lectin domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.