Rabbit Anti-MMP1 antibody |
反应物种(预测) |
Cow |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,心血管,细胞生物,信号转导,细胞骨架, |
标签 |
Array |
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Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-MMP-1 (bs-0424R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27/54kD
Observed band size: 50kD
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Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-1 Polyclonal Antibody, Unconjugated(bs-0424R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Heart (Rabbit) Lysate at 30 ug
Primary: Anti-MMP-1 (bs-0424R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 24 kD
Observed band size: 27 kD
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Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- MMP-1 (bs-0424R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
RRID:AB_10858058
产品名称:Rabbit Anti-MMP1 antibody
别名: 27 kDa interstitial collagenase; CLGN; CLG; collagenase, fibroblast; Fibroblast collagenase; Interstitial collagenase; MMP 1; MMP-1; MMP; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metall
中文名称:基质金属蛋白酶-1抗体
英文名称:Rabbit Anti-MMP1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞外基质,分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human MMP1
抗原表位:401-469/469
SWISS:P03956
Gene ID :4312
Human Gene ID:4312
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurot
Subunit:Interacts with HIV-1 Tat.
Subcellular Location:Secreted, extracellular space, extracellular matrix (Probable).
Post-translational modifications:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Similarity:Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.