Rabbit Anti-HSP90 beta antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-HSP90 beta antibody

多克隆 | SKU：bs-0135R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0135R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-HSP90 beta antibody
反应物种（预测）	Chicken,Dog,Pig,Cow
产品应用（已验证）	WB,IHC,ICC
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100,
研究领域	肿瘤,细胞生物,免疫学,信号转导,细胞凋亡
标签	Array

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSP90 beta) Polyclonal Antibody, Unconjugated (bs-0135R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP90 beta) polyclonal Antibody, Unconjugated (bs-0135R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Primary: Anti-HSP90 beta (bs-0135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84-90 kD
Observed band size: 84 kD
Sample:
HepG2 Cell Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- HSP90 beta (bs-0135R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kD
Observed band size: 90kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP90 beta) polyclonal Antibody, Unconjugated (bs-0135R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

产品信息

RRID：AB_10852591
产品名称：Rabbit Anti-HSP90 beta antibody
别名： HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Hea
中文名称：热休克蛋白90β/HSP90 β 抗体
英文名称：Rabbit Anti-HSP90 beta antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human HSP90 beta
抗原表位：635-724/724
SWISS：P08238
Gene ID ：3326
Human Gene ID：3326

产品介绍

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
Function：Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activ
Subunit：Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
Subcellular Location：Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications：Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
Similarity：Belongs to the heat shock protein 90 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.