| Rabbit Anti-NKG2D antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=2μg/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学 |
| 标签 |
Array |
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Blank control (blue line): Mouse thymus cells (blue).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control:SW480.
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (bs-0938R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
RRID：AB_10854448
产品名称：Rabbit Anti-NKG2D antibody
别名： NKG2-D type II integral membrane protein; KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD 314; CD314 a
中文名称：NK细胞受体2D（CD314）抗体
英文名称：Rabbit Anti-NKG2D antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from rat NKG2D
抗原表位：121-219/219
抗原细胞定位：Extracellular
SWISS：O70215
Gene ID ：24934
Human Gene ID：22914
This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]
Function：Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymph
Subunit：Homodimer.
Subcellular Location：Membrane; Single-pass type II membrane protein.
Tissue Specificity：Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
Similarity：Contains 1 C-type lectin domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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