Rabbit Anti-GRP78 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-GRP78 antibody

多克隆 | SKU：bs-1219R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-1219R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-GRP78 antibody
产品应用（已验证）	WB,IHC
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
研究领域	肿瘤,细胞生物
标签	Array

Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: U-87MG (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Hyperpituitarism (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 74 kD
Sample:
U87mg(Human) Cell Lysate at 30 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti- GRP78 (bs-1219R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: Intestinal(Mouse) Lysate at 30ug;
Primary: Anti-GRP78 (bs-1219R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 78 kD
Observed band size :78 kD
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(bs-1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

产品信息

RRID：AB_10856907
产品名称：Rabbit Anti-GRP78 antibody
别名： BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobuli
中文名称：葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体
英文名称：Rabbit Anti-GRP78 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human GRP78
抗原表位：251-360/654
SWISS：P11021
Gene ID ：3309
Human Gene ID：3309

产品介绍

The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Function：Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subcellular Location：Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Similarity：Belongs to the heat shock protein 70 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.