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Mouse Anti-Bax antibody
多克隆  |   SKU:bs-0127M

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货号:bs-3351R
¥1280
订购号:bs-0127M
¥1098.00-2900.00
货期:现货
Mouse Anti-Bax antibody
反应物种(预测)

Dog,Pig,Cow,Sheep

产品应用(已验证)

WB,IHC,ICC

产品应用(可尝试)

IF,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100,

研究领域

细胞凋亡

标签

Array

  • Tissue/cell: SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
  • Sample:
    Lane 1: Recombinant human Bax Protein (full length) Overexpression E.coli Lysate (Cat#: bs-41243P) at 2 ug
    Primary: Anti-Bax (bs-0127M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 70 kD
    Observed band size: 73 kD
  • Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
  • Sample: Placenta(Mouse)lysates, 30ug;
    Primary: Anti-Bax (bs-0127M) at 1:300;
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
    Predicted band size : 21 kD
    Observed band size : 23 kD
  • Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

产品信息

RRID:AB_10855515
产品名称:Mouse Anti-Bax antibody
别名: apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isof
中文名称:Bax抗体
英文名称:Mouse Anti-Bax antibody
抗体来源: Mouse
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human Bax
抗原表位:84-175/192
SWISS:Q07812
Gene ID :581
Human Gene ID:581

产品介绍

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].
Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, lea
Subunit:Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E
Subcellular Location:Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins an
Tissue Specificity:Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lu
Similarity:Belongs to the Bcl-2 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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