Rabbit Anti-TGF beta Receptor I antibody |
反应物种(预测) |
Cow |
产品应用(已验证) |
WB,IHC,IF,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
研究领域 |
肿瘤,神经生物学,信号转导,生长因子和激素,细胞膜受体 |
标签 |
Array |
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Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Small intestine (Rat) Lysate at 40 ug
Primary: Anti- TGF beta Receptor I (bs-0638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 58 kD
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-TGF Beta R1/TGFBR1 Polyclonal Antibody, Unconjugated(bs-0638R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control:U937.
Primary Antibody (green line): Rabbit Anti-TGF beta Receptor I antibody (bs-0638R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:AB_10855338
产品名称:Rabbit Anti-TGF beta Receptor I antibody
别名: TGF-beta receptor type-1; TGFBR1; TGF Beta R1; AAT 5; AAT5; Activin A receptor type II like kinase 53kDa; Activin receptor like kinase 5; ACVRLK 4; ACVRLK4; ALK 5; ALK5; Serine/threonine protein kinase receptor R4; SKR 4; SKR4; TbetaR I; TGF beta receptor
中文名称:转移生长因子β受体1抗体
英文名称:Rabbit Anti-TGF beta Receptor I antibody
中文别名:TGF-βRⅠ; TGFβRⅠ; TGF-βR Ⅰ; TGF-β RⅠ.
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human TGF beta Receptor I
抗原表位:301-400/501
抗原细胞定位:Extracellular
SWISS:P36897
Gene ID :7046
Human Gene ID:7046
The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Function:On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional
Subunit:Homodimer; in the endoplasmic reticulum but also at the cell membrane. Heterohexamer; TGFB1, TGFB2 and TGFB3 homodimeric ligands assemble a functional receptor composed of two TGFBR1 and TGFBR2 heterodimers to form a ligand-receptor heterohexamer. The res
Subcellular Location:Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction.
Tissue Specificity:Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.
Post-translational modifications:Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region.
Similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.