| Rabbit Anti-PDGF-B antibody |
| 反应物种（预测） |
Dog,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
心血管,信号转导,生长因子和激素 |
| 标签 |
Array |
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Sample:
Stomach (Rat) Lysate at 40 ug
Primary: Anti-PDGF-B (bs-0185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD
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Blank control (blue line):Hela(blue).
Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (bs-0185R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: Lung (Rat) Lysate at 40 ug
Lane 6: Huvec (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Lane 8: MCF-7 (Human) Cell Lysate at 30 ug
Lane 9: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-PDGF-B (bs-0185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 28 kD
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Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated(bs-0071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10859010
产品名称：Rabbit Anti-PDGF-B antibody
别名： PDGF beta; PDGF-B; Platelet-derived growth factor B-chain; PDGF AB + BB; Becaplermin; c sis; FLJ1285; Oncogene SIS; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B chain; PDGF subunit A; PDGF subunit B; PDGF1; PDGF2; PDGFA; PDGFB; PDGFR1; Platelet derived gr
中文名称：血小板源性生长因子-B抗体
英文名称：Rabbit Anti-PDGF-B antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PDGF-B
抗原表位：155-210/241
SWISS：P01127
Gene ID ：5155
Human Gene ID：5155
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. [SUBUNIT] Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. Belongs to the PDGF/VEGF growth factor family.
Function：Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericy
Subunit：Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PD
Subcellular Location：Secreted. Note=Released by platelets upon wounding.
Tissue Specificity：Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
DISEASE：Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
Similarity：Belongs to the PDGF/VEGF growth factor family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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