| Rabbit Anti-AQP1 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,信号转导,通道蛋白,新陈代谢, |
| 标签 |
Array |
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Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP-1 Polyclonal Antibody, Unconjugated(bs-1506R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Blank control: 293T (blue).
Primary Antibody:Rabbit Anti-AQP1 antibody(bs-1506R), Dilution: 1μg in 100μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1506R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-AQP1 (bs-1506R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
RRID：AB_10852587
产品名称：Rabbit Anti-AQP1 antibody
别名： AQP 1; AQP-1; AQP1_HUMAN; Aquaporin-1; Aquaporin-CHIP; Channel like integral membrane protein 28 kDa; MGC26324; Water channel protein CHIP 29; AQP CHIP; AQP1; Aquaporin CHIP; Aquaporin1; Aquaporin 1; Channel forming integral protein 28kDa; CHIP 28; CHIP2
中文名称：水通道蛋白1抗体
英文名称：Rabbit Anti-AQP1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human AQP1
抗原表位：181-269/269
抗原细胞定位：Cytoplasmic
SWISS：P29972
Gene ID ：358
Human Gene ID：358
Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Function：Forms a water-specific channel that provides the plasmamembranes of red cells and kidney proximal tubules with highpermeability to water, thereby permitting water to move in thedirection of an osmotic gradient.
Subunit：Homotetramer. Interacts with EPHB2; involved in endolymphproduction in the inner ear (By similarity).
Subcellular Location：Membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Similarity：Belongs to the MIP/aquaporin (TC 1.A.8) family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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