| Rabbit Anti-PARP1 antibody |
| 反应物种（预测） |
Rat,Dog,Cow |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg/Test,IF=1:100-500, |
| 研究领域 |
染色质和核信号,细胞凋亡 |
| 标签 |
Array |
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Sample:
Hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- PARP (bs-2138R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
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Blank control:293T.
Primary Antibody (green line): Rabbit Anti-PARP1 antibody (bs-2138R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Protein:HepG2 lysate at 30ug;
Primary: Anti-PARP (bs-2138R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:111 kD
Observed band size:111 kD
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Paraformaldehyde-fixed, paraffin embedded (mouse intestines); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (bs-2138R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Blank control (blue line): HL60 cells (blue).
Primary Antibody (green line): Rabbit Anti- PARP antibody (bs-2138R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug
Lane 2: HL60 (Human) Cell Lysate at 30 ug
Lane 3: K562 (Human) Cell Lysate at 30 ug
Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-PARP (bs-2138R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
-
Sample:
Hela Cell (Human) Lysate at 30 ug
Primary: Anti- PARP (bs-2138R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
-
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti- PARP (bs-2138R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
RRID：AB_10855907
产品名称：Rabbit Anti-PARP1 antibody
别名： ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family
中文名称：多腺苷二磷酸多聚酶抗体(N端)
英文名称：Rabbit Anti-PARP1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PARP
抗原表位：201-300/1014
SWISS：P09874
Gene ID ：142
Human Gene ID：142
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].
Function：Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligat
Subunit：Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and Z
Subcellular Location：Mitochondrion outer membrane; Single-pass membrane protein.
Nucleus membrane; Single-pass membrane protein.
Endoplasmic reticulum membrane; Single-pass membrane protein.
Nucleus.
Post-translational modifications：Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription r
Similarity：Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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