Rabbit Anti-Caspase-12 antibody |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞凋亡 |
标签 |
Array |
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Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
Lane 2: Muscle (Rat) Tissue Lysate at 40 ug
Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 47/40 kD
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Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kD
Observed band size: 46 kD
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Tissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(bs-1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-1105R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
RRID:AB_10858192
产品名称:Rabbit Anti-Caspase-12 antibody
别名: UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12 (gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207032; OTTHUM
中文名称:半胱胺酸蛋白酶蛋白-12抗体
英文名称:Rabbit Anti-Caspase-12 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from the middle of human Caspase-12
抗原表位:201-300/420
SWISS:O08736
Gene ID :100506742
Human Gene ID:100506742
Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011].
Function:Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
Tissue Specificity:Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
Similarity:Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.