| Rabbit Anti-MMP13 antibody |
| 反应物种（预测） |
Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500, |
| 研究领域 |
肿瘤,心血管,信号转导,细胞骨架,细胞外基质, |
| 标签 |
Array |
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Tissue/cell: rat articular cartilage; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-0575R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-0575R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Sample:
Ovary (Rat)Lysate at 40 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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MG63 (Human)Lysate at 30 ug
U 2OS(Human)Lysate at 30 ug
MDA-MB-231(Human)Lysate at 30 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 60 kD
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Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
RRID：AB_10857557
产品名称：Rabbit Anti-MMP13 antibody
别名： CLG 3; CLG3; Collagenase 3; Collagenase3; MMP13; MMP 13; MMP-13; Matrix Metalloproteinase 13; MMP 13; MMP13_HUMAN.
中文名称：基质金属蛋白酶13抗体
英文名称：Rabbit Anti-MMP13 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human MMP13
抗原表位：201-300/471
SWISS：P45452
Gene ID ：4322
Human Gene ID：4322
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].
Function：Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
Subcellular Location：Secreted, extracellular space, extracellular matrix (Probable).
Tissue Specificity：Seems to be specific to breast carcinomas.
DISEASE：Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bow
Similarity：Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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