| Rabbit Anti-LDL receptor antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,ICC,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg/Test,ICC=1:100, |
| 研究领域 |
肿瘤,免疫学 |
| 标签 |
Array |
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Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (bs-0705R) ;
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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Blank control：A431.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (bs-0705R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody:Goat anti-rabbit IgG-AF647,Dilution: 1μg /test.
Protocol
A431 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (bs-0705R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:Human colon Lysate at 45ug;
Primary: Anti-LDL receptor (bs-0705R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 92kD
Observed band size : 92kD
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Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (bs-0705R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
K562(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-LDL receptor (bs-0705R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 120 kD
RRID：AB_10856023
产品名称：Rabbit Anti-LDL receptor antibody
别名： Low-density lipoprotein receptor precursor; LDL-R; LDL R; FH; FHC; LDL R; LDLR; Low Density Lipoprotein Receptor; Low density lipoprotein receptor familial hypercholesterolemia; LDLR_HUMAN.
中文名称：低密度脂蛋白受体抗体
英文名称：Rabbit Anti-LDL receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human LDL-R
抗原表位：781-860/860
抗原细胞定位：Cytoplasmic
SWISS：P01130
Gene ID ：3949
Human Gene ID：3949
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.
Function：Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as
Subunit：Interacts with LDLRAP1. Interacts with SNX17. Interacts with HCV E1/E2 heterodimer. Interacts with HIV-1 Tat.
Subcellular Location：Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. Note=Found distributed from the plasma membrane to intracellular compartments
Tissue Specificity：Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as
Post-translational modifications：N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Similarity：Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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