| Rabbit Anti-WNT5A antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,信号转导 |
| 标签 |
Array |
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated(bs-1948R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Images provided the Independent Validation Program (badge number 029641)Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (bs-1948R) at 1:250 at room temperature overnight followed by conjugation to secondary antibody.
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Sample:Ovary(Mouse) Lysate at 30 ug
Primary: Anti-WNT5A (bs-1948R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 47 kD
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Sample:Uters(Mouse) Lysate at 30 ug
Primary: Anti-WNT5A (bs-1948R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 47 kD
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-WNT5A antibody (bs-1948R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Embryo (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: Uterus (Rat) Lysate at 40 ug
Lane 5: Ovary (Rat) Lysate at 40 ug
Lane 6: Vas deferens (Rat) Lysate at 40 ug
Primary: Anti-WNT5A (bs-1948R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
RRID：AB_10856979
产品名称：Rabbit Anti-WNT5A antibody
别名： wingless-related MMTV integration site 5A; hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt-5a; WNT 5A protein precursor; WNT5A protein precursor; WNT5A_HUMAN.
中文名称：信号通路Wnt5a抗体
英文名称：Rabbit Anti-WNT5A antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human WNT5A
抗原表位：301-381/381
SWISS：P41221
Gene ID ：7474
Human Gene ID：7474
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Function：Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the ca
Subunit：Interacts with PORCN. Interacts with WLS.
Subcellular Location：Secreted, extracellular space, extracellular matrix.
Tissue Specificity：Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
Post-translational modifications：Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
Glycosylation is necessary for secretion but not for activity.
DISEASE：Defects in WNT5A are the cause of Robinow syndrome autosomal dominant (DRS) [MIM:180700]. A disease characterized by short-limb dwarfism, costovertebral segmentation defects and abnormalities of the head, face and external genitalia. The clinical signs ar
Similarity：Belongs to the Wnt family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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