| Rabbit Anti-Dopamine D2 Receptor antibody |
| 产品应用（已验证） |
WB,IHC,IF |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,细胞膜受体,G蛋白偶联受体,G蛋白信号, |
| 标签 |
Array |
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Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D2 Receptor (bs-1008R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60kD
Observed band size: 60kD
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D2 Receptor) Polyclonal Antibody, Unconjugated (bs-1008R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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Sample:
Lane 1: BV2 (Mouse) Cell Lysate at 30 ug
Lane 2: RSC96 (Rat) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Lane 4: U251 (Human) Cell Lysate at 30 ug
Lane 5: U87MG (Human) Cell Lysate at 30 ug
Primary: Anti- Dopamine D2 Receptor (bs-1008R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D2 Receptor) Polyclonal Antibody, Unconjugated (bs-1008R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10858064
产品名称：Rabbit Anti-Dopamine D2 Receptor antibody
别名： D2DR; D2 dopamine receptor; D2R; Dopamine receptor D2; Dopamine Receptor D2L; DRD 2; DRD2; DRD2_HUMAN.
中文名称：多巴胺受体D2抗体
英文名称：Rabbit Anti-Dopamine D2 Receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human DRD2
抗原表位：201-300/443
抗原细胞定位：Cytoplasmic
SWISS：P14416
Gene ID ：1813
Human Gene ID：1813
7 transmembrane receptor (rhodopsin family). This family contains, amongst other G-protein-coupled receptors (GCPRs), members of the opsin family, which have been considered to be typical members of the rhodopsin superfamily. They share several motifs, mainly the seven transmembrane helices, GCPRs of the rhodopsin superfamily. All opsins bind a chromophore, such as 11-cis-retinal. The function of most opsins other than the photoisomerases is split into two steps: light absorption and G-protein activation. Photoisomerases, on the other hand, are not coupled to G-proteins - they are thought to generate and supply the chromophore that is used by visual opsins.
Function：This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular Location：Cell membrane.
DISEASE：Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnorma
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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