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Rabbit Anti-GFP antibody
多克隆  |   SKU:bs-0639R

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货号:bs-3351R
¥1280
订购号:bs-0639R
¥680.00-4000.00
货期:现货
Rabbit Anti-GFP antibody
反应物种(预测)

Species independent

产品应用(已验证)

WB

产品应用(可尝试)

IF

推荐稀释比例

WB=1:20000-50000,IF=1:100-500,

研究领域

细胞生物,免疫学

标签

Array

  • Sample:
    GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
    Primary: Anti-GFP (bs-0639R) at 1/1000 ~ 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 28 kD
    Observed band size: 28 kD
  • Sample:
    GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug
    Primary: Anti-GFP (bs-0639R) at 1/1000 ~ 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 28 kD
    Observed band size: 28 kD
  • Sample:
    GFP-Tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
    Primary:
    Anti-GFP (bs-0639R) at 1/20000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 28 kD
    Observed band size: 30 kD
  • Sample:
    Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
    Lane 2: Control (-) E.coli Lysate at 4 ug
    Primary: Anti-GFP (bs-0639R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 28 kD
    Observed band size: 28 kD

产品信息

RRID:AB_10856207
产品名称:Rabbit Anti-GFP antibody
别名: Green Fluorecent protein.
中文名称:绿色荧光蛋白抗体
英文名称:Rabbit Anti-GFP antibody
中文别名:绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP);
抗体来源: Rabbit
克隆类型:多克隆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from belt jellyfish GFP
抗原表位:151-238/238
SWISS:P42212
Gene ID :107331116
Human Gene ID:107331116

产品介绍

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Function:Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Subunit:Monomer.
Tissue Specificity:Photocytes.
Post-translational modifications:Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing oth
Similarity:Belongs to the GFP family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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