| Rabbit Anti-c-fos antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
IHC,ICC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,转录调节因子,肿瘤细胞生物标志物, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-10172R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-10172R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-10172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-10172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (c-fos) polyclonal Antibody, Unconjugated (bs-10172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID：RRID
产品名称：Rabbit Anti-c-fos antibody
别名： Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogen
中文名称：c-fos抗体
英文名称：Rabbit Anti-c-fos antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human c-fos
抗原表位：145-260/380
SWISS：P01100
Gene ID ：2353
Human Gene ID：2353
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].
Function：Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a mul
Subunit：Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activat
Subcellular Location：Nucleus.
Post-translational modifications：Phosphorylated in the C-terminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with
Similarity：Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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