| Rabbit Anti-Myc tag antibody |
| 反应物种（预测） |
Species independent |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:1000-5000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,染色质和核信号,信号转导,细胞周期蛋白,转录调节因子,细胞分化, |
| 标签 |
Array |
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Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C-Myc Polyclonal Antibody, Unconjugated(bs-0842R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-Myc tag (bs-0842R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
RRID：AB_10881637
产品名称：Rabbit Anti-Myc tag antibody
别名： avian myelocytomatosis viral oncogene homolog;
bHLHe39;
c-Myc;
C-Myc-Tag;
class E basic helix-loop-helix protein 39;
MRTL;
MYC;
Myc Epitope Tag;
myc proto-oncogene protein;
myc-related translation/localization regulatory factor;
oncogene c-Myc;
中文名称：c-Myc tag标签抗体
英文名称：Rabbit Anti-Myc tag antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated C-EQKLISEEDL (human Proto-oncogene c-Myc)
SWISS：P01106
Gene ID ：4609
Human Gene ID：4609
Epitope tags are useful for the labeling and detection of proteins using
immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
Subunit：Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FB
Subcellular Location：Nucleus, nucleoplasm. Nucleus, nucleolus.
Similarity：Contains 1 basic helix-loop-helix (bHLH) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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