| Rabbit Anti-CD147 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,合成与降解,细胞表面分子, |
| 标签 |
Array |
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Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD147/TCSF/Emmprin Polyclonal Antibody, Unconjugated(bs-0684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Liver(Mouse) Lysate at 40 ug
Placenta(Mouse) Lysate at 40 ug
HepG2 Cell Lysate at 40 ug
Primary: Anti-CD147 (bs-0684R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 60 kD
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Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: U251 (Human) Cell Lysate at 30 ug
Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
Primary: Anti-CD147 (bs-0684R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-60 kD
Observed band size: 60 kD
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Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD
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Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-CD147 (Bs-0684R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD147) Polyclonal Antibody, Unconjugated (bs-0684R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control (blue line): MCF7 (blue).
Primary Antibody (green line):Rabbit Anti-CD147 antibody(bs-0684R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10856796
产品名称：Rabbit Anti-CD147 antibody
别名： TCSF; Emmprin; 5A11 antigen; 5F7; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; BSG; CD 147; CD147 antigen; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; M6 leuko
中文名称：细胞外基质金属蛋白酶诱导因子抗体
英文名称：Rabbit Anti-CD147 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TCSF
抗原表位：301-385/385
抗原细胞定位：Cytoplasmic
SWISS：P35613
Gene ID ：682
Human Gene ID：682
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function：Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8
Subunit：Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monoc
Subcellular Location：Cell membrane; Single-pass type I membrane protein. Melanosome.
Tissue Specificity：Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Post-translational modifications：N-glycosylated.
Similarity：Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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