| Rabbit Anti-BMP7 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,细胞凋亡,生长因子和激素,转录调节因子 |
| 标签 |
Array |
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Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-BMP7 (bs-2242R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
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Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti-BMP7 (bs-2242R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47kD
Observed band size: 49/40 kD
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Tissue/cell: rabbit alveolar bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BMP7 Polyclonal Antibody, Unconjugated(bs-2242R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-BMP7 (bs-2242R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47 kD
Observed band size: 47,50kD
RRID：AB_10858045
产品名称：Rabbit Anti-BMP7 antibody
别名： bone morphogenetic protein 7 precursor; osteogenic protein 1; BMP-7; OP-1; BMP 7; Bone morphogenic protein 7; Eptotermin alfa; OP 1; OP1; BMP7_HUMAN.
中文名称：骨形态发生蛋白7抗体
英文名称：Rabbit Anti-BMP7 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human BMP7
抗原表位：293-350/431
SWISS：P18075
Gene ID ：655
Human Gene ID：655
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq].
Function：Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Subunit：Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1.
Subcellular Location：Secreted.
Tissue Specificity：Expressed in the kidney and bladder. Lower levels seen in the brain.
Post-translational modifications：Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).
Similarity：Belongs to the TGF-beta family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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