| Rabbit Anti-BTG2 antibody |
| 反应物种（预测） |
Rat,Pig,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
细胞生物,染色质和核信号,神经生物学,细胞凋亡 |
| 标签 |
Array |
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Tissue/cell: mouse small intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BTG2 Polyclonal Antibody, Unconjugated(bs-0031R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BTG2 Polyclonal Antibody, Unconjugated(bs-0031R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
HepG2 (human)cell Lysate at 40 ug
Hcclm3 (human)cell Lysate at 40 ug
Primary: Anti- BTG2 (bs-0031R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17kD
Observed band size: 17 kD
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Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-BTG2 (bs-0031R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 16 kD
RRID：AB_10856537
产品名称：Rabbit Anti-BTG2 antibody
别名： BTG2_HUMAN; Protein BTG2; BTG family member 2; NGF-inducible anti-proliferative protein PC3; pheochromacytoma cell-3; nerve growth factor-inducible anti-proliferative; B-cell translocation gene 2; BTG2; TIS21; PC3; TIS21.
中文名称：B细胞迁移基因2抗体
英文名称：Rabbit Anti-BTG2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human BTG2
抗原表位：31-110/158
SWISS：P78543
Gene ID ：7832
Human Gene ID：7832
BTG2 is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. BTG2 is involved in the regulation of the G1/S transition of the cell cycle. It modulates transcription regulation mediated by ESR1(referenced from Entrez Gene). BTG2 expression is induced in vivo during neurogenesis, and the gene is transiently expressed in vitro in rat pheochromocytoma PC12 cells after induction of neuronal differentiation by addition of nerve growth factor (NGF); suggesting that BTG2 is functionally significant during the neuronal differentiation process (PMID: 12360398).
Function：Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenyltion in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involve
Subunit：Interacts with PRKCABP. Interacts with CNOT7 and CNOT8; indicative for an asscociation with the CCR4-NOT complex. Interacts with PIN1, inducing mitochondrial depolarization.
Post-translational modifications：Phosphorylated at Ser-147 by MAPK1/ERK2 and MAPK3/ERK1, and at Ser-149 by MAPK14, leading to PIN1-binding and mitochondrial depolarization.
Similarity：Belongs to the BTG family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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