| Rabbit Anti-XBP1 antibody |
| 反应物种（预测） |
Chicken,Cow |
| 产品应用（已验证） |
WB,ICC,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
转录调节因子,表观遗传学, |
| 标签 |
Array |
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U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with XBP1 Antibody(bs-1668R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
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Sample:
Lane 1: Mouse Skin Lysates
Lane 2: Mouse Testis Lysates
Lane 3: Rat Skin Lysates
Lane 4: Rat Testis Lysates
Primary: Anti-XBP1 (bs-1668R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29/40kDa
Observed band size: 29/40kDa
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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (bs-1668R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:
Large intestine (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- XBP1 (bs-1668R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29/40 kD
Observed band size: 29 kD
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Sample:
Lane1: Embryo(Mouse) Lysate at 30 ug
Lane2: Liver (Mouse) Lysate at 30 ug
Primary: Anti-XBP-1(bs-1668R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 29kD
Observed band size : 29kD
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Sample: Liver(Mouse) Lysate at 40 ug
Primary: Anti- XBP1 (bs-1668R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29kD
Observed band size: 29 kD
RRID：AB_10855632
产品名称：Rabbit Anti-XBP1 antibody
别名： X box-binding protein-1; XBP-1; XBP2; Tax responsive element binding protein 5; TREB5; X box binding protein 2; XBP 1; XBP2; XBP1_HUMAN; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; X-box-binding protein 1 in isoform 2
中文名称：细胞核转录因子X盒结合蛋白抗体
英文名称：Rabbit Anti-XBP1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human XBP-1
抗原表位：51-150/261
SWISS：P17861
Gene ID ：7494
Human Gene ID：7494
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]
Function：Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) t
Subcellular Location：Nucleus.
DISEASE：Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature
Similarity：Belongs to the bZIP family.
Contains 1 bZIP domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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