| Rabbit Anti-ADGRE1 antibody |
| 反应物种（预测） |
Rat,Pig,GuineaPig |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=2μg/Test, |
| 研究领域 |
免疫学,生长因子和激素,G蛋白偶联受体,糖蛋白,G蛋白信号, |
| 标签 |
Array |
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Positive control: mouse Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA;
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody : rabbit Anti-EMR1 bs-7058R;
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.
-
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 (bs-7058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
-
Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 (bs-7058R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
-
Blank control: Mouse brain.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (bs-7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (bs-7058R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-EMR1 antibody (bs-7058R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-EMR1 (bs-7058R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
RRID：RRID
产品名称：Rabbit Anti-ADGRE1 antibody
别名： F4/80; Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1;
中文名称：表皮生长因子样激素受体1（EMR1）抗体
英文名称：Rabbit Anti-ADGRE1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human ADGRE1
抗原表位：701-800/886
抗原细胞定位：Extracellular
SWISS：Q14246
Gene ID ：2015
Human Gene ID：2015
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function：Could be involved in cell-cell interactions.
Subunit：Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain.
Subcellular Location：Cell membrane.
Tissue Specificity：Wide expression; increased levels in peripheral blood mononuclear cells.
Similarity：Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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