| Mouse Anti-AFP(A2) antibody |
| 产品应用（已验证） |
WB,IF |
| 产品应用（可尝试） |
IHC,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤 |
| 标签 |
Array |
-
Sample: AFP recombinded protein lysate at 100ng;
Primary: Anti-AFP(A4) (bsm-1622M) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-mouse IgG(bs-0296G-HRP) at 1: 5000 dilution;
Predicted band size: 65 kD
Observed band size: 65 kD
-
Sample: AFP recombinded protein lysate at 100ng;
Primary: Anti-AFP(A4) (bsm-1622M) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-mouse IgG(bs-0296G-HRP) at 1: 5000 dilution;
Predicted band size: 65 kD
Observed band size: 65 kD
-
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (bsm-1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
-
Sample:
Lane 1: Rat Embryo Lysates
Lane 2: Rat Placenta Lysates
Primary: Anti-AFP(A2) (bsm-1621M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65kDa
Observed band size: 65kDa
RRID：AB_10855834
产品名称：Mouse Anti-AFP(A2) antibody
别名： Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
中文名称：甲胎蛋白单克隆抗体（包被）
英文名称：Mouse Anti-AFP(A2) antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：AFP protein
抗原表位：full length
SWISS：P02771
Gene ID ：174
Human Gene ID：174
Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Subunit：Dimeric and trimeric forms have been found in addition to the monomeric form.
Subcellular Location：Secreted.
Tissue Specificity：Plasma. Synthesized by the fetal liver and yolk sac.
Similarity：Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏