| Rabbit Anti-Amylin antibody |
| 反应物种（预测） |
Chicken,Dog,Rabbit |
| 产品应用（已验证） |
IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
心血管,信号转导,糖尿病,新陈代谢, |
| 标签 |
Array |
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amylin Polyclonal Antibody, Unconjugated(bs-0057R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amylin Polyclonal Antibody, Unconjugated(bs-0057R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amylin) Polyclonal Antibody, Unconjugated (bs-0057R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855323
产品名称：Rabbit Anti-Amylin antibody
别名： Islet amyloid polypeptide; IAPP; DAP; Diabetes associated peptide; IAP; Insulinoma amyloid peptide.
中文名称：糖尿病相关肽/胰岛淀粉样肽抗体
英文名称：Rabbit Anti-Amylin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Amylin
抗原表位：1-37/89
SWISS：P10997
Gene ID ：3375
Human Gene ID：3375
CAS：3
Amylin is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the assosciation of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta amyloid (Abeta) assosciated with Alzheimer's disease, can induce apoptotic cell death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.
Function：Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Subunit：Interacts with IDE and INS. Can form homodimers. Interaction with INS inhibits homodimerization and fibril formation.
Subcellular Location：Secreted.
Post-translational modifications：Amyloid fibrils are degraded by IDE.
Similarity：Belongs to the calcitonin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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