| Rabbit Anti-IL6ST/CD130 antibody |
| 产品应用（已验证） |
WB,IHC,IF,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1µg/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,信号转导,细胞膜受体 |
| 标签 |
Array |
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Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-6R Beta/CD130/gp130 Polyclonal Antibody, Unconjugated(bs-1459R) 1:300, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Blank control: Raji(blue).
Primary Antibody:Rabbit Anti-CD34 antibody(bs-1459R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1459R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1459R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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Tissue/cell: Human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-6R Beta/CD130/gp130 Polyclonal Antibody, Unconjugated(bs-1459R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Lung(Mouse) Lysate at 30 ug
Primary: Anti- CD130 (bs-1459R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 99 kD
Observed band size: 130 kD
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Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD130) Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD130) Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:lung(mouse) Lysate at 40 ug
Primary: Anti- CD130 (bs-1459R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99kD
Observed band size: 130 kD
RRID：AB_10855104
产品名称：Rabbit Anti-IL6ST/CD130 antibody
别名： IL-6R Beta; Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-be
中文名称：白细胞介素6受体β/CD130抗体
英文名称：Rabbit Anti-IL6ST/CD130 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human IL6R beta
抗原表位：651-750/917
抗原细胞定位：Cytoplasmic
SWISS：P40189
Gene ID ：3572
Human Gene ID：3572
CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Function：Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, an
Subunit：Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a h
Subcellular Location：Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Tissue Specificity：Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Post-translational modifications：Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.
Similarity：Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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