| Rabbit Anti-GnRHR antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC,IF |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
信号转导,生长因子和激素 |
| 标签 |
Array |
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Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-GnRHR (bs-1464R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62-70 kD
Observed band size: 63 kD
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
RRID：AB_10855060
产品名称：Rabbit Anti-GnRHR antibody
别名： Gonadotropin-releasing hormone receptor; GH1; Lhrhr; GnRH receptor; gnrh-r; GnRHR; GNRHR1; Gonadotropin releasing hormone receptor; GRHR; leutinizing-releasing hormone receptor; lh-rh; LHRHR; LRHR; luteinizing hormone releasing hormone receptor; GNRHR_HUM
中文名称：促性腺激素释放激素受体抗体
英文名称：Rabbit Anti-GnRHR antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human GnRHR
抗原表位：201-328/328
抗原细胞定位：Cytoplasmic
SWISS：P30968
Gene ID ：2798
Human Gene ID：2798
Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. Activin A has been shown to stimulate the synthesis of GnRHR, illustrating a possible mechanism for the modulation of gonadotropin responsiveness to GnRH.
Function：Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association w
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Pituitary, ovary, testis, breast and prostate but not in liver and spleen.
DISEASE：Hypogonadotropic hypogonadism 7 with or without anosmia (HH7) [MIM:146110]: A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no othe
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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