| Rabbit Anti-Neurturin antibody |
| 反应物种（预测） |
Rat,Dog,Cow |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
发育生物学,神经生物学 |
| 标签 |
Array |
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Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurturin Polyclonal Antibody, Unconjugated(bs-0073R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Placenta(Mouse)lysate at 30 ug;
Brain(Mouse) lysate at 30 ug;
Primary: Anti- Neurturin (bs-0073R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 21 kD
Observed band size: 21 kD
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Sample:
Lane 1: Human K562 cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Neurturin (bs-0073R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21kDa
Observed band size: 21kDa
RRID：AB_10855987
产品名称：Rabbit Anti-Neurturin antibody
别名： Neurturin precursor; Neurturin; NRTN; NTN; NRTN_HUMAN.
中文名称：神经营养因子抗体
英文名称：Rabbit Anti-Neurturin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Neurturin
抗原表位：151-197/197
SWISS：Q99748
Gene ID ：4902
Human Gene ID：4902
Human Neurturin (NTN) and Human GDNF comprise a family of TGF-beta related neurotrophic factor which have trophic influences on a variety of neuronal populations. Neurturin promotes the survival of certain sympathetic and sensory neurins through interaction with distinct set of GDNF-like receptors
Function：Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Subunit：Homodimer; disulfide-linked.
Subcellular Location：Secreted.
DISEASE：Note=Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intr
Similarity：Belongs to the TGF-beta family. GDNF subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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