| Rabbit Anti-SNAI2 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学,信号转导,转录调节因子 |
| 标签 |
Array |
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Sample:
Lane1: Heart (Rat) Lysate at 30 ug
Lane2: Liver (Rat) Lysate at 30 ug
Primary: Anti-SNAI2/SLUG (bs-1382R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 30kD
Observed band size : 30kD
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SNAI2 Polyclonal Antibody, Unconjugated(bs-1382R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10856900
产品名称：Rabbit Anti-SNAI2 antibody
别名： SLUG; Neural crest transcription factor Slug; Protein snail homolog 2; Slug (chicken homolog) zinc finger protein; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI-2; SNAI2_HUMAN; Snail 2; Snail homolog 2; S
中文名称：锌指转录因子Slug抗体
英文名称：Rabbit Anti-SNAI2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Slug
抗原表位：5-120/268
SWISS：O43623
Gene ID ：6591
Human Gene ID：6591
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008].
Function：Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occluding (OCLN) and subsequent oncogenic transformation of epithelial cells.
Subunit：Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).
Subcellular Location：Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active spli
Tissue Specificity：Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermi
Post-translational modifications：GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.
DISEASE：Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) . WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of d
Similarity：Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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