| Rabbit Anti-PTGES2 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500, |
| 研究领域 |
肿瘤,转录调节因子 |
| 标签 |
Array |
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Blank control:A549.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PTGES2 antibody (bs-2639R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10860215
产品名称：Rabbit Anti-PTGES2 antibody
别名： C9orf15;
FLJ14038;
Gamma interferon activated transcriptional element binding factor 1;
GATE binding factor 1;
GBF 1;
GBF1;
Membrane associated prostaglandin E synthase 2;
MGC11289;
Microsomal prostaglandin E synthase 2;
mPGES 2;
mPGES-2;
PGES2
中文名称：前列腺素E合成酶2抗体
英文名称：Rabbit Anti-PTGES2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from mouse PTGES2
抗原表位：281-384/384
抗原细胞定位：Cytoplasmic
SWISS：Q8BWM0
Gene ID ：96979
Human Gene ID：80142
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq].
Function：Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction w
Subunit：Homodimer. May interact with CEBPB.
Subcellular Location：Golgi apparatus membrane; Single-pass membrane protein. Nucleus. Note=According to PubMed:12050152, some fraction may be nuclear. Prostaglandin E synthase 2 truncated form: Cytoplasm. Note=Synthetized as a Golgi membrane-bound protein, which is further cl
Tissue Specificity：Widely expressed. Expressed in brain, heart, liver, colon and lung.
Similarity：Belongs to the GST superfamily.
Contains 1 glutaredoxin domain.
Contains 1 GST C-terminal domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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