Rabbit Anti-Phospho-GCN2 (Thr899) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

首页 > 产品 > 一抗 > 产品信息

磷酸化抗体

Rabbit Anti-Phospho-GCN2 (Thr899) antibody

多克隆 | SKU：bs-3155R

说明书

联系我们

打印此页面

货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
相关产品
相关文献

订购号:bs-3155R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-Phospho-GCN2 (Thr899) antibody
反应物种（预测）	Rat,Dog,Cow,Horse
产品应用（已验证）	FCM
产品应用（可尝试）	WB,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/Test,
研究领域	肿瘤,细胞生物,染色质和核信号,神经生物学,信号转导,激酶和磷酸酶
标签	Array

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：AB_10857865
产品名称：Rabbit Anti-Phospho-GCN2 (Thr899) antibody
别名： GCN2 (phospho T899); p-GCN2 (phospho T899); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2
中文名称：磷酸化蛋白激酶GCN2蛋白抗体
英文名称：Rabbit Anti-Phospho-GCN2 (Thr899) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899
抗原表位：HL(p-T)GM
SWISS：Q9P2K8
Gene ID ：440275
Human Gene ID：440275

产品介绍

GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.
Function：Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
Tissue Specificity：Widely expressed.
Post-translational modifications：Autophosphorylated on threonine residues.
Similarity：Belongs to the protein kinase superfamily.
Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.