| Rabbit Anti-Connexin-40 antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
心血管,信号转导,细胞粘附分子, |
| 标签 |
Array |
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Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti- Connexin-40 antibody(bs-1050R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-1050R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1050R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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Tissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Connexin-40 Polyclonal Antibody, Unconjugated(bs-1050R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Connexin-40 Polyclonal Antibody, Unconjugated(bs-1050R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Connexin-40 ) Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-Connexin-40 (bs-1050R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
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Sample:
Lane 1: Mouse Kidney Lysates
Lane 2: Rat Kidney Lysates
Primary: Anti-Connexin-40 (bs-1050R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40kDa
Observed band size: 40kDa
RRID：AB_10857532
产品名称：Rabbit Anti-Connexin-40 antibody
别名： connexin 40; connexin40; CX40; Gap junction alpha 5 protein; gap junction protein alpha 5 40kD (connexin 40); gap junction protein alpha 5; GJA5; MGC11185; CXA5_HUMAN; Gap junction alpha-5 protein; Connexin-40; Cx40.
中文名称：间隙连接蛋白40抗体
英文名称：Rabbit Anti-Connexin-40 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Connexin-40
抗原表位：301-358/358
抗原细胞定位：Cytoplasmic
SWISS：P36382
Gene ID ：2702
Human Gene ID：2702
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]
Function：One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit：A connexon is composed of a hexamer of connexins.
Subcellular Location：Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
DISEASE：Familial atrial standstill (FAS) [MIM:108770]: Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of
Similarity：Belongs to the connexin family. Alpha-type (group II) subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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