| Rabbit Anti-PI 3 Kinase Class 3 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=3ug/Test,IF=1:100-500, |
| 研究领域 |
免疫学,信号转导,激酶和磷酸酶 |
| 标签 |
Array |
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PI 3 Kinase Class 3 Polyclonal Antibody, Unconjugated(bs-4159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
brain(mouse) Lysate at 40 ug
Primary: Anti-PI 3 Kinase Class 3 (bs-4159R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98kD
Observed band size: 98 kD
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Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-PI 3 Kinase Class 3 antibody (bs-4159R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (bs-4159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_11086765
产品名称：Rabbit Anti-PI 3 Kinase Class 3 antibody
别名： PI 3 Kinase Class 3; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphoinositide 3 kinase class 3; PI3 kinase type 3; PI3K type 3; PIK3C3; PtdIns 3 kinase type
中文名称：磷脂酰肌醇激酶3催化亚单位3抗体
英文名称：Rabbit Anti-PI 3 Kinase Class 3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PIK3C3/PI3 kinase type 3
抗原表位：301-400/887
SWISS：Q8NEB9
Gene ID ：5289
Human Gene ID：5289
PI 3 Kinase Class 3 is a member of the PI3/PI4-kinase family. It is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes. It is ubiquitously expressed, with a highest expression in skeletal muscle.
Function：Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for th
Subunit：Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UV
Subcellular Location：Midbody. Late endosome.
Tissue Specificity：Ubiquitously expressed, with a highest expression in skeletal muscle.
Similarity：Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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