| Rabbit Anti-5HT1A Receptor antibody |
| 反应物种（预测） |
Dog,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学,信号转导,转录调节因子,细胞膜受体,G蛋白偶联受体,G蛋白信号, |
| 标签 |
Array |
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Tissue/cell: rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5HT1A Receptor Polyclonal Antibody, Unconjugated(bs-1124R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Protein: HepG2 lyates(hu);
Primary: Rabbit Anti- 5HT1A Receptor (bs-1124R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:46 kD
Observed band size:56 kD
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Protein: Brain(Mouse) Lysate at 40 ug
Primary: Rabbit Anti- 5HT1A Receptor (bs-1124R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:46 kD
Observed band size:56 kD
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Sample:
Jurkat(Human) Cell Lysate at 40 ug
Primary: Anti-5HT1A Receptor (bs-1124R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Mouse Lymph Lysates
Lane 4: Rat Brain Lysates
Lane 5: Rat Cerebellum Lysates
Primary: Anti-5HT1A Receptor (bs-1124R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46kDa
Observed band size: 46kDa
RRID：AB_10858513
产品名称：Rabbit Anti-5HT1A Receptor antibody
别名： 5HT1A Receptor; HTR1A; 5-HTR1A; 5 HT 1A; 5 HT receptor 1A; 5 HT1A; 5 HT1A receptor; 5 hydroxytryptamine (serotonin) receptor 1A; 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled; 5 hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; 5-hydroxytryp
中文名称：5-羟色胺受体1A抗体
英文名称：Rabbit Anti-5HT1A Receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human 5-HTR1A
抗原表位：301-400/422
抗原细胞定位：Cytoplasmic
SWISS：P19327
Gene ID ：3350
Human Gene ID：3350
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012].
Function：This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase a
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Similarity：Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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