| Rabbit Anti-ADAMTS1 antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,心血管,细胞生物,信号转导,细胞类型标志物,细胞骨架, |
| 标签 |
Array |
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Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human 293T cell Lysates
Lane 3: Human U87-MG cell Lysates
Lane 4: Human U251 cell Lysates
Primary: Anti-ADAMTS1 (bs-1208R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106kDa
Observed band size: 80kDa
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10858673
产品名称：Rabbit Anti-ADAMTS1 antibody
别名： A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin like and metalloprotease with thrombospondin type 1 motif 1; A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1; ADAM metallopeptidase
中文名称：整合素样金属蛋白酶与凝血酶1型抗体
英文名称：Rabbit Anti-ADAMTS1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from mouse ADAMTS1
抗原表位：851-967/967
SWISS：P97857
Gene ID ：11504
Human Gene ID：9510
ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function.
Function：Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachex
Subcellular Location：Secreted > extracellular space > extracellular matrix.
Post-translational modifications：The precursor is cleaved by a furin endopeptidase.
Similarity：Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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