| Rabbit Anti-TGF Beta 1+2+3 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Sheep,GuineaPig |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,信号转导,生长因子和激素,转录调节因子 |
| 标签 |
Array |
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated(bs-4538R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Spleen (Rabbit) Lysate at 40 ug
Primary: Anti-TGF Beta 1+2+3 (bs-4538R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 12.8/44 kD
Observed band size: 44 kD
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Sample: Stomach (mouse) Lysate at 40 ug
Primary: Anti- TGF Beta 1+2+3 (bs-4538R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44kD
Observed band size: 44 kD
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Blank control: K562.
Primary Antibody (green line): Rabbit Anti-TGF Beta 1+2+3 antibody (bs-4538R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_11107525
产品名称：Rabbit Anti-TGF Beta 1+2+3 antibody
别名： TGF Beta 1/2/3; TGFβ1; TGFβ2; TGFβ3; CED; Diaphyseal dysplasia 1 progressive; DPD 1; DPD1; TGF beta 1; TGF beta; TGF beta1; TGF beta2; TGF beta3; TGF-beta 1; TGF-beta 2; TGF-beta 3; TGFB 1; TGFB; TGFB1; TGFB2; TGFB3; Transforming growth factor; Transformi
中文名称：转化生长因子β1抗体
英文名称：Rabbit Anti-TGF Beta 1+2+3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TGF Beta 1
抗原表位：301-350/390
SWISS：P01137
Gene ID ：7040
Human Gene ID：7040
The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta)1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs); glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis.
Function：Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an impo
Subunit：Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (L
Subcellular Location：Secreted, extracellular space, extracellular matrix.
Tissue Specificity：Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Post-translational modifications：Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
DISEASE：Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The d
Similarity：Belongs to the TGF-beta family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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