| Rabbit Anti-ILK-1 antibody |
| 反应物种（预测） |
Chicken,Dog,Cow |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,神经生物学,信号转导 |
| 标签 |
Array |
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Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ILK-1 Polyclonal Antibody, Unconjugated(bs-0317R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lung (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-ILK-1 (bs-0317R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
RRID：AB_10855394
产品名称：Rabbit Anti-ILK-1 antibody
别名： 59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 2.7.11.1; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked
中文名称：整合素连接激酶-1抗体
英文名称：Rabbit Anti-ILK-1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human ILK-1
抗原表位：301-400/452
SWISS：Q13418
Gene ID ：3611
Human Gene ID：3611
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin linked kinase (ILK), interacts with the cytoplasmic domain of beta 1 Integrin. ILK encodes a predicted 451 amino acid protein, with an apparent molecular weight of 59 kDa. The ILK protein is a serine/threonine protein kinase with 4 ankyrin like repeats. ILK regulates integrin mediated signal transduction.
The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
Function：Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points
Subunit：Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. Interacts (via ANK
Subcellular Location：Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity：Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Post-translational modifications：Autophosphorylated on serine residues.
Similarity：Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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