| Rabbit Anti-HCN4 antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
心血管,免疫学,通道蛋白 |
| 标签 |
Array |
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Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-HCN4 (bs-1691R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD
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Sample:
Lane 1: Mouse Testis Lysates
Lane 2: Rat Testis Lysates
Lane 3: Human HL-60 cell Lysates
Lane 4: Human MCF-7 cell Lysates
Lane 5: Human A549 cell Lysates
Primary: Anti-HCN4 (bs-1691R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129kDa
Observed band size: 129kDa
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Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HCN4 Polyclonal Antibody, Unconjugated(bs-1691R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10857774
产品名称：Rabbit Anti-HCN4 antibody
别名： HCN 4;Hyperpolarization activated cyclic nucleotide gated potassium channel 4; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4;Hyperpolarization activated Cyclic Nucleotide-gated channel 4; HCN4_HUMAN.
中文名称：环化核苷酸调控阳离子通道蛋白亚型4
英文名称：Rabbit Anti-HCN4 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human HCN4
抗原表位：501-600/1203
抗原细胞定位：Cytoplasmic
SWISS：Q9Y3Q4
Gene ID ：10021
Human Gene ID：10021
HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
Function：Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediat
Subunit：The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
Subcellular Location：Membrane; Multi-pass membrane protein.
Tissue Specificity：Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus.
DISEASE：Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dy
Similarity：Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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