| Rabbit Anti-FGF1 antibody |
| 反应物种（预测） |
Rat,Sheep |
| 产品应用（已验证） |
IHC,ICC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,生长因子和激素 |
| 标签 |
Array |
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Blank control: A431.
Primary Antibody (green line): Rabbit Anti-FGF1 antibody(bs-0229R)
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF1) Polyclonal Antibody, Unconjugated (bs-0229R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF1) Polyclonal Antibody, Unconjugated (bs-0229R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10859171
产品名称：Rabbit Anti-FGF1 antibody
别名： Acidic fibroblast growth factor;
Acidic-fibroblast growth factor;
aFGF;
Beta endothelial cell growth factor;
Beta-endothelial cell growth factor;
ECGF;
ECGF beta;
ECGF-beta;
ECGFA;
ECGFB;
Endothelial Cell Growth Factor alpha;
Endothelial Cell G
中文名称：酸性成纤维细胞生长因子抗体
英文名称：Rabbit Anti-FGF1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human FGF1
抗原表位：16-80/155
SWISS：P05230
Gene ID ：2246
Human Gene ID：2246
FGF1 also designated Heparin-binding growth factor 1 precursor (HBGF-1) ; Acidic fibroblast growth factor (aFGF). heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Subunit si monomer.FGF1 belongs to the heparin-binding growth factors family. may play a role in neurite outgrowth; may regulate cell differentiation in the nervous system; may act in synergy with fibronectin to enhance neuronal cell adhesion.
Function：The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Subunit：Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 a
Subcellular Location：Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Note=Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A
Tissue Specificity：Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
Post-translational modifications：In the nucleus, phosphorylated by PKC/PRKCD.
Similarity：Belongs to the heparin-binding growth factors family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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