Rabbit Anti-CYP11A1 antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse,Rabbit,Sheep,53/57 |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,转录调节因子,线抗体, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (bs-10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Cell: HepG2.
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10099R) on HepG2(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (bs-10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:Testis(Mouse)Lysate at 40 ug
Primary: Anti-CYP11A1(bs-10099R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53/57 kD
Observed band size: 53/57 kD
RRID:AB_2751005
产品名称:Rabbit Anti-CYP11A1 antibody
别名: Cholesterol 20 22 desmolase;
Cholesterol desmolase;
Cholesterol monooxygenase (side chain cleaving);
Cholesterol side chain cleavage enzyme;
Cholesterol side chain cleavage enzyme mitochondrial;
Cholesterol side-chain cleavage enzyme;
CP11A_HUMAN;
中文名称:细胞色素P450 11A1抗体
英文名称:Rabbit Anti-CYP11A1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC
抗原表位:41-140/521
SWISS:P05108
Gene ID :1583
Human Gene ID:1583
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Function:Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Subcellular Location:Mitochondrion membrane.
DISEASE:Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adr
Similarity:Belongs to the cytochrome P450 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.