| Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,信号转导,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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Blank control:U937.
Primary Antibody (green line): Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody (bs-5587R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Rat Thymus Lysates
Lane 2: Human HT-1080 cell Lysates
Primary: Anti-phospho-PI 3 Kinase p110 delta (Tyr524)(bs-5587R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kDa
Observed band size: 115 kDa
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Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI 3 Kinase p110 delta (Tyr524) Polyclonal Antibody, Unconjugated (bs-5587R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_11099488
产品名称：Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody
别名： PI 3 Kinase p110 delta (phospho Tyr524); PIK3CD(phospho Y524); PI3 Kinase p110 delta; p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidyl
中文名称：磷酸化磷脂酰肌醇激酶催化亚单位D抗体
英文名称：Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human PIK3CD around the phosphorylation site of Tyr524
抗原表位：EL(p-Y)EH
SWISS：O00329
Gene ID ：5293
Human Gene ID：5293
PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p110. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K. Four isoforms of p110 has been found; the alpha, beta, gamma, and the delta subunit. The delta isoform is predominantly expressed in leukocytes and has been shown to interact with p85 and GTP-bound Ras via its SH2/SH3 domain.
Subunit：Heterodimer of a catalytic subunit PIK3CD and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with ERAS.
Tissue Specificity：Expressed predominantly in leukocytes.
Similarity：Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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