| Rabbit Anti-Frizzled 5 antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,干细胞,G蛋白偶联受体,细胞表面分子,G蛋白信号, |
| 标签 |
Array |
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Frizzled 5 (bs-2930R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
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Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Frizzled 5 antibody (bs-2930R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Mouse Liver Lysates
Lane 2: Mouse Brain Lysates
Lane 3: Human HepG2 cell Lysates
Lane 4: Human K562 cell Lysates
Lane 5: Human A549 cell Lysates
Primary: Anti-Frizzled 5 (bs-2930R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kDa
Observed band size: 62kDa
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Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Frizzled 5 Polyclonal Antibody, Unconjugated(bs-2930R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Frizzled 5 Polyclonal Antibody, Unconjugated(bs-2930R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Images provided the Independent Validation Program (badge number 28751)Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-Frizzled 5 Polyclonal Antibody (bs-2930R) at 1:250 room temperature overnight followed by conjugation to secondary antibody.
RRID：AB_11061004
产品名称：Rabbit Anti-Frizzled 5 antibody
别名： C2orf31; DKFZP434E2135; Frizzled homolog 5; Frizzled-5; Frizzled5; Fz 5; Fz-5; Fz5; FZD 5; hFz5; FZD5; FZD5_HUMAN; FzE 5; FzE5; HFZ 5; HFZ5; MGC129692; Seven transmembrane receptor frizzled 5; Uncharacterized protein C2orf31; Wnt receptor.
中文名称：Wnt信号受体蛋白抗体
英文名称：Rabbit Anti-Frizzled 5 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Frizzled 5
抗原表位：51-150/585
抗原细胞定位：Extracellular
SWISS：Q13467
Gene ID ：7855
Human Gene ID：7855
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 5 protein is believed to be the receptor for the Wnt5A ligand. Frizzled 5 has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. Frizzled 5 was cloned from a retina cDNA library.
Function：Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt
Subunit：Interacts with GOPC (By similarity).
Subcellular Location：Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
Post-translational modifications：Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Similarity：Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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